Quesada MD, Giménez M, Molinos S, Fernández G, Sánchez MD, Rivelo R et al (2010) Performance of VITEK-2 compact and overnight MicroScan panels for direct identification and susceptibility testing of Gram-negative bacilli from positive FAN BacT/ALERT blood culture bottles. īruins MJ, Bloembergen P, Ruijs GJHM, Wolfhagen MJHM (2004) Identification and susceptibility testing of Enterobacteriaceae and Pseudomonas aeruginosa by direct inoculation from positive BACTEC blood culture bottles into Vitek 2. Hogan CA, Watz N, Budvytiene I, Banaei N (2019) Rapid antimicrobial susceptibility testing by VITEK®2 directly from blood cultures in patients with Gram-negative rod bacteremia. ĭoern CD (2018) The slow March toward rapid phenotypic antimicrobial susceptibility testing: are we there yet? J Clin Microbiol 56:e01999–e01917. J Infect Dev Ctries 4(12):822–827ĭubourg G, Raoult D, Fenollar F (2019) Emerging methodologies for pathogen identification in bloodstream infections: an update. J Clin Microbiol 27(6):1342–1345īarman P, Sengupta S, Singh S (2010) Study of a novel method to assist in early reporting of sepsis from the microbiology laboratory. Trenholme GM, Kaplan RL, Karakusis PH, Stine T, Fuhrer J, Landau W et al (1989) Clinical impact of rapid identification and susceptibility testing of bacterial blood culture isolates. Kerremans JJ, Verboom P, Stijnen T, Hakkaart-van Roijen L, Goessens W, Verbrugh HA et al (2008) Rapid identification and antimicrobial susceptibility testing reduce antibiotic use and accelerate pathogen-directed antibiotic use. J Clin Microbiol 32(7):1757–1762īarenfanger J, Drake C, Kacich G (1999) Clinical and financial benefits of rapid bacterial identification and antimicrobial susceptibility testing. This method has the main advantage of providing reliable results 1 day earlier, being a simple, fast, and cheap method for identification and antimicrobial susceptibility testing results from positive blood cultures.ĭoern GV, Vautour R, Gaudet M, Levy B (1994) Clinical impact of rapid in vitro susceptibility testing and bacterial identification. aeruginosa and amoxicillin-clavulanate, ciprofloxacine, gentamicine, and cotrimoxazole in E. mirabilis, betalactams antibiotics (except second- and third-generation cephalosporines) showed a good correlation, and also a good correlation was found for ciprofloxacine and gentamicine in P. pneumoniae, except amoxicillin-clavulanate and piperacillin-tazobactam. We found 46 very major errors, but globally the results showed a good correlation with the standard method, particularly favorable for E. Overall, categorical agreement was 92.86%. About 96.5% were correctly identified with the direct method. Conventional and direct identification and AST were performed simultaneously by both methods in 1070 blood cultures, and 9106 MICs were determinated. We describe a simple and inexpensive method to obtain faster AST with MicroScan system (Beckman Coulter) directly from positive blood cultures. Conventional blood culture methods are the gold standard diagnostic test to guide management of patient with sepsis, but the conventional process requires at least 12 to 24 h after the blood culture has been flagged as positive due to requirement for pure colonies. Shortening the turnaround time of antimicrobial susceptibility testing (AST) of bacteria permits a significant reduction of patient morbidity, mortality, and cost.
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